The Rat Ang1-7 (Angiotensin 1-7) ELISA Kit is a specialized assay designed to accurately quantify the levels of Angiotensin 1-7 in rat biological samples. Angiotensin 1-7 is a biologically active peptide hormone that plays a crucial role in the regulation of blood pressure, cardiovascular function, and fluid balance. This hormone is an essential component of the renin-angiotensin system, contributing to vasodilation, anti-inflammatory effects, and cardiovascular protection in rats. By measuring Angiotensin 1-7 levels, researchers can gain valuable insights into the physiological effects of this critical peptide in rats, including its impact on blood pressure regulation, cardiovascular health, and renal function. The Rat Ang1-7 ELISA Kit provides researchers with a reliable and precise method for studying the role of Angiotensin 1-7 in various physiological processes and disease models related to cardiovascular health and renal function in rats.
With exceptional sensitivity and specificity, this ELISA kit ensures accurate and reproducible results, enabling researchers to conduct in-depth investigations into the biological functions and regulatory mechanisms of Angiotensin 1-7 in rats. Manufactured under strict quality control standards, the kit offers robust performance and user-friendly protocols, making it an excellent choice for research studies focused on the renin-angiotensin system and cardiovascular physiology in rats.
Product Name:
Rat Ang1-7 (Angiotensin 1-7) ELISA Kit
SKU:
AEES00693
Target:
Rat Ang1-7 (Angiotensin 1-7)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang1-7. During the reaction, Ang1-7 in the sample or standard competes with a fixed amount of Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
89-101
84-97
Average (%)
102
94
90
1:4
Range (%)
94-105
91-105
83-94
Average (%)
99
97
89
1:8
Range (%)
93-108
86-100
87-100
Average (%)
100
91
92
1:16
Range (%)
99-115
85-100
87-101
Average (%)
105
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.05
150.48
436.34
46.79
150.27
427.32
Standard deviation
2.53
8.29
13.61
3.13
7.62
16.37
C V (%)
5.05
5.51
3.12
6.69
5.07
3.83
Sample type &Sample volume:
; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang1-7 concentrations in .
Specificity:
This kit recognizes Ang1-7 in samples. No significant cross-reactivity or interference between Ang1-7 and analogues was observed.
