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Rat Amyloid Precursor Protein (APP) ELISA Kit

SKU:
RTDL00102
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08592
Sensitivity:
0.49ng/mL
Range:
1.25-80ng/mL
ELISA Type:
Sandwich
Synonyms:
AAA,ABeta,ABPP,AD1,APPI,CVAP,PN2,CTFgamma,Peptidase Nexin-II Alzheimer Disease,Peptidase Nexin-II,Amyloid Beta(A4)Precursor Protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Amyloid Precursor Protein (APP) ELISA Kit

The Rat Amyloid Precursor Protein (APP) ELISA Kit is specifically designed for the precise measurement of rat amyloid precursor protein levels in various biological samples including serum, plasma, and tissue lysates. This kit provides accurate and reliable results with high sensitivity and specificity, making it an ideal tool for researchers studying neurodegenerative disorders such as Alzheimer's disease.Amyloid precursor protein is a key player in the pathogenesis of Alzheimer's disease, as it is cleaved to produce amyloid-beta peptides that aggregate and form plaques in the brain.

By accurately quantifying APP levels, researchers can better understand the mechanisms underlying Alzheimer's disease and potentially identify novel therapeutic targets for intervention.Overall, the Rat Amyloid Precursor Protein (APP) ELISA Kit offers a valuable tool for studying neurodegenerative disorders and advancing research in the field of Alzheimer's disease.

Product Name:Rat Amyloid Precursor Protein (APP) ELISA Kit
Product Code:RTDL00102
Size:96 Assays
Target:APP
Synonyms:AAA, ABeta, ABPP, AD1, APPI, CVAP, PN2, CTFgamma, Peptidase Nexin-II Alzheimer Disease, Peptidase Nexin-II, Amyloid Beta(A4)Precursor Protein
Detection Method:Sandwich
Range:1.25-80ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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