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Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit

SKU:
RTDL00084
Product Type:
ELISA Kit
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
VAP1,HPAO,SSAO,Membrane primary amine oxidase,Vascular Adhesion Protein 1,Semicarbazide-Sensitive Amine Oxidase,Copper-Containing Amine Oxygen Oxidoreductase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit

The Rat Amine Oxidase Copper-containing 3 (AOC3) ELISA Kit is a reliable and sensitive tool for the precise measurement of AOC3 levels in rat samples including serum, plasma, and tissue lysates. This kit boasts high specificity and accuracy, ensuring consistent and trustworthy results for a variety of research applications.AOC3 is an important enzyme that plays a key role in amine metabolism, contributing to the regulation of oxidative stress and inflammation. Dysregulation of AOC3 has been implicated in various diseases such as liver dysfunction, cardiovascular disorders, and neurological conditions, making it a valuable biomarker for understanding these pathological processes and exploring potential therapeutic interventions.

Overall, the Rat AOC3 ELISA Kit provides researchers with a valuable tool for investigating the role of AOC3 in physiological and pathological conditions, offering a reliable and efficient method for quantifying AOC3 levels in rat samples.

Product Name:Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit
Product Code:RTDL00084
Size:96 Assays
Target:AOC3
Synonyms:VAP1, HPAO, SSAO, Membrane primary amine oxidase, Vascular Adhesion Protein 1, Semicarbazide-Sensitive Amine Oxidase, Copper-Containing Amine Oxygen Oxidoreductase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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