The Rat AlphaFP (Alpha Fetoprotein) CLIA Kit is a specialized assay designed for the precise measurement of Alpha Fetoprotein levels in rat serum, plasma, and tissue extracts. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Alpha Fetoprotein is a key biomarker associated with liver development and function, as well as a marker for certain types of cancer.
By accurately measuring Alpha Fetoprotein levels, researchers can gain valuable insights into liver diseases, hepatocellular carcinoma, and other health conditions. Overall, the Rat AlphaFP CLIA Kit offers a reliable and efficient solution for studying Alpha Fetoprotein dynamics in rat models, contributing to a better understanding of liver-related disorders and the development of potential therapeutic interventions.
Product Name:
Rat alphaFP (Alpha-Fetoprotein) CLIA Kit
SKU:
RTES00091
Target:
Rat alphaFP (Alpha-Fetoprotein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat αFP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat αFP. You can calculate the concentration of Rat αFP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
87-97
101-118
Average (%)
105
92
108
1:4
Range (%)
90-103
87-98
96-110
Average (%)
96
92
101
1:8
Range (%)
100-119
99-110
88-99
Average (%)
108
105
94
1:16
Range (%)
91-102
96-113
102-118
Average (%)
97
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
98-111
106
Cell culture media (n=5)
95-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.65
467.75
1537.88
211.72
497.04
1443.96
Standard deviation
18.41
49.72
171.63
20.83
50.85
164.47
C V (%)
9.41
10.63
11.16
9.84
10.23
11.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat αFP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat αFP in samples. No significant cross-reactivity or interference between Rat αFP and analogues was observed.
