Rat Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
The Rat Alpha-2-HS-Glycoprotein (AHSG) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of AHSG levels in rat serum, plasma, and cell culture supernatants. AHSG, also known as fetuin-A, is a multifunctional glycoprotein involved in various biological processes such as cell signaling, inflammation, and regulation of mineral metabolism.This ELISA kit provides researchers with a reliable and reproducible method for studying the role of AHSG in a variety of research areas, including diabetes, cardiovascular disease, and osteoporosis.
By accurately quantifying AHSG levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.Overall, the Rat Alpha-2-HS-Glycoprotein (AHSG) ELISA Kit is a valuable tool for researchers looking to investigate the biological functions of AHSG and its potential implications in various diseases. Its high sensitivity and specificity make it a reliable choice for a wide range of research applications.
Product Name:
Rat Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
