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Rat Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) ELISA Kit

SKU:
RTDL00054
Product Type:
ELISA Kit
Sensitivity:
0.133ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ATQ1,PDE,EPD,Antiquitin,Alpha-Aminoadipic Semialdehyde Dehydrogenase,Delta1-Piperideine-6-Carboxylate Dehydrogenease,P6c Dehydrogenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) ELISA Kit

The Rat ALDH7A1 ELISA Kit is specifically designed for the precise measurement of ALDH7A1 levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for various research applications.ALDH7A1, a member of the aldehyde dehydrogenase enzyme family, plays a critical role in the metabolism of aldehydes and can be linked to a variety of diseases and disorders.

Studying ALDH7A1 levels can provide valuable insights into conditions such as metabolic disorders, neurodegenerative diseases, and various genetic disorders. This ELISA kit serves as a valuable tool in understanding ALDH7A1 biology and potential therapeutic interventions.

Product Name:Rat Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) ELISA Kit
Product Code:RTDL00054
Size:96 Assays
Target:ALDH7A1
Synonyms:ATQ1, PDE, EPD, Antiquitin, Alpha-Aminoadipic Semialdehyde Dehydrogenase, Delta1-Piperideine-6-Carboxylate Dehydrogenease, P6c Dehydrogenase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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