The Rat ALB (Albumin) ELISA Kit is a specialized assay designed for the quantitative detection of albumin levels in rat biological samples. Albumin is a key plasma protein that plays a crucial role in regulating osmotic pressure, maintaining pH balance, and transporting various substances in the bloodstream. It is synthesized by the liver and is essential for overall metabolic function and fluid homeostasis.
Measuring albumin levels can provide valuable insights into liver function, renal health, and nutritional status. Changes in albumin levels have been associated with liver and kidney diseases, malnutrition, and various systemic disorders. Therefore, accurate quantification of albumin is crucial for assessing overall health and disease states. The Rat ALB ELISA Kit offered by Assay Genie provides researchers with a reliable and sensitive tool for the quantitative analysis of albumin levels in biological samples. This kit is manufactured under stringent quality control standards to ensure robust performance, exceptional sensitivity, and specificity, making it an excellent choice for research applications aimed at studying albumin dynamics in various physiological and pathological conditions.
Product Name:
Rat ALB (Albumin) ELISA Kit
SKU:
AEES00390
Target:
Rat ALB (Albumin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 μg/mL
Detection range:
3.13-200 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALB. You can calculate the concentration of Rat ALB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
83-95
98-111
Average (%)
104
90
104
1:4
Range (%)
97-111
86-100
96-112
Average (%)
103
93
103
1:8
Range (%)
97-112
82-91
93-107
Average (%)
105
87
100
1:16
Range (%)
95-111
80-91
91-104
Average (%)
102
86
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.9
25.5
76.0
9.8
23.3
78.5
Standard deviation
0.5
1.2
3.6
0.5
1.2
2.7
C V (%)
5.05
4.71
4.74
5.1
5.15
3.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ALB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed.
