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Rat Adrenocorticotropic Hormone Receptor (ACTHR) ELISA Kit

SKU:
RTDL00016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G3V848
Sensitivity:
0.234ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
MC2R,MC2-R,Melanocortin 2 Receptor,ACTH Receptor,Adrenocorticotropin receptor,Melanocortin receptor 2
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat Adrenocorticotropic Hormone Receptor (ACTHR) ELISA Kit

The Rat Adrenocorticotropic Hormone Receptor (ACTHR) ELISA Kit is a reliable and accurate tool for measuring levels of ACTHR in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.ACTHR is a vital receptor involved in hormone signaling, particularly in the regulation of adrenal gland function and stress response. Understanding the role of ACTHR can provide valuable insights into various physiological and pathological processes, including stress disorders, immune responses, and metabolic disorders.

With its comprehensive features and proven performance, the Rat ACTHR ELISA Kit is an essential tool for researchers studying the intricate mechanisms of hormone signaling and its implications in health and disease. Get the insights you need with confidence and accuracy using this advanced ELISA kit.

Product Name:Rat Adrenocorticotropic Hormone Receptor (ACTHR) ELISA Kit
Product Code:RTDL00016
Size:96 Assays
Target:ACTHR
Synonyms:MC2R, MC2-R, Melanocortin 2 Receptor, ACTH Receptor, Adrenocorticotropin receptor, Melanocortin receptor 2
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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