The Rat Adiponectin ELISA Kit is specifically designed for the quantitative detection of Adiponectin (also known as ADP or Acrp30) levels in various rat biological samples. Adiponectin is a vital adipokine that plays a significant role in metabolic processes, including insulin sensitivity, glucose regulation, and lipid metabolism.
As a key regulator of energy homeostasis, Adiponectin is involved in modulating various cellular functions related to obesity, diabetes, cardiovascular diseases, and inflammation. Accurate measurement of Adiponectin levels using this ELISA kit provides researchers with valuable insights into the intricate mechanisms underlying metabolic regulation and its impact on physiological pathways. By quantifying Adiponectin with exceptional sensitivity and specificity, this kit enables detailed investigations into the role of Adiponectin in metabolic disorders, insulin resistance, and obesity-related complications. The Rat Adiponectin ELISA Kit offers reliable and reproducible results, allowing researchers to study the intricate relationship between Adiponectin levels and metabolic diseases. Manufactured under stringent quality control standards, this kit ensures robust performance and user-friendly protocols, making it an excellent choice for research studies focusing on adipokine biology, metabolic disorders, and related therapeutic strategies.
Product Name:
Rat ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
AEES00528
Target:
Rat ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. You can calculate the concentration of Rat ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-96
88-97
99-109
Average (%)
92
92
105
1:4
Range (%)
101-108
86-97
91-101
Average (%)
104
91
97
1:8
Range (%)
88-100
95-104
101-110
Average (%)
93
99
105
1:16
Range (%)
88-100
98-111
88-101
Average (%)
92
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
85-93
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.91
10.17
40.58
4.17
11.09
42.27
Standard deviation
0.29
0.51
1.83
0.23
0.53
1.88
C V (%)
5.91
5.01
4.51
5.52
4.78
4.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ADP/Acrp30 in samples. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed.
