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Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

SKU:
RTDL00053
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O35112
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD166,MEMD,Activated Leucocyte Cell Adhesion Molecule
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

The Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit is a powerful tool for accurately measuring levels of ALCAM in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it an essential resource for a variety of research applications.ALCAM is a critical molecule involved in cell adhesion and migration, playing a key role in immune response and inflammation.

Dysregulation of ALCAM has been linked to various diseases and disorders, including autoimmune diseases, cancer, and neurological conditions, making it a valuable biomarker for understanding these conditions and developing new therapeutic approaches.With the Rat ALCAM ELISA Kit, researchers can confidently investigate the role of ALCAM in disease pathology and progression, paving the way for innovative discoveries and potential treatment strategies.

Product Name:Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit
Product Code:RTDL00053
Size:96 Assays
Target:ALCAM
Synonyms:CD166, MEMD, Activated Leucocyte Cell Adhesion Molecule
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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