Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit
The Rat ADAM10 (A Disintegrin and Metalloprotease 10) ELISA Kit is a highly reliable and accurate tool for the measurement of ADAM10 levels in rat samples. This kit is specifically designed for use with rat serum, plasma, and tissue homogenates, providing researchers with a valuable resource for studying the role of ADAM10 in various biological processes.ADAM10 is a key enzyme involved in the proteolytic processing of a variety of proteins, including cell adhesion molecules and cytokines. Its dysregulation has been linked to numerous diseases, such as Alzheimer's disease, cancer, and inflammatory conditions.
By accurately measuring ADAM10 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.With its high sensitivity and specificity, the Rat ADAM10 ELISA Kit offers researchers a reliable and reproducible method for quantifying ADAM10 levels in rat samples. This makes it an essential tool for studies investigating the role of ADAM10 in disease pathogenesis and for the development of novel therapeutic interventions.
Product Name:
Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit
Product Code:
RTDL00017
Size:
96 Assays
Target:
ADAM10
Synonyms:
CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
