The Rat Aβ1-40 (Amyloid Beta 1-40) ELISA Kit is a specialized assay designed for the quantitative measurement of Aβ1-40 levels in various biological samples collected from rat models.
Aβ1-40 is a key peptide fragment that plays a significant role in the pathogenesis of Alzheimer's disease, particularly in the formation of amyloid plaques, a hallmark of this neurodegenerative disorder. This ELISA kit provides researchers with a precise and reliable method to quantify Aβ1-40 levels, enabling detailed investigations into the role of this peptide in Alzheimer's disease pathophysiology and neurodegeneration. By accurately measuring Aβ1-40 with exceptional sensitivity and specificity, researchers can gain valuable insights into the mechanisms underlying Alzheimer's disease and explore potential therapeutic strategies targeting Aβ. Manufactured under stringent quality control standards, the Rat Aβ1-40 ELISA Kit ensures robust performance, accurate results, and ease of use, making it an indispensable tool for research studies focusing on Alzheimer's disease, amyloid pathology, and neurodegenerative disorders in rat models.
Product Name:
Rat Aβ1-40 (Amyloid Beta 1-40) ELISA Kit
SKU:
AEES00544
Target:
Rat Aβ1-40 (Amyloid Beta 1-40)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Aβ1-40. You can calculate the concentration of Rat Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
90-101
92-105
Average (%)
97
93
100
1:4
Range (%)
92-104
90-103
99-109
Average (%)
96
97
103
1:8
Range (%)
97-109
93-103
94-103
Average (%)
104
96
97
1:16
Range (%)
100-111
95-105
88-98
Average (%)
105
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
97-108
101
Cell culture media (n=5)
94-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.79
115.21
417.92
40.74
106.44
457.42
Standard deviation
2.35
5.66
22.19
2.06
4.96
22.69
C V (%)
5.37
4.91
5.31
5.06
4.66
4.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Aβ1-40 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Aβ1-40 in samples. No significant cross-reactivity or interference between Rat Aβ1-40 and analogues was observed.
