The RASSF7 Polyclonal Antibody (PACO58468) is a valuable tool for research focused on RASSF7, a tumor suppressor gene involved in cell cycle regulation and apoptosis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RASSF7 protein, this antibody enables the detection and analysis of RASSF7 expression in a variety of cell types, making it well-suited for studies in cancer biology and tumor development.
RASSF7 is a key player in the regulation of cellular processes that prevent abnormal cell growth and contribute to the maintenance of genomic stability. Its role as a tumor suppressor gene highlights its importance in understanding cancer progression and identifying potential therapeutic targets. Research on RASSF7 may provide insights into the mechanisms underlying tumorigenesis and aid in the development of novel anti-cancer strategies that target this gene.
Western Blot. Positive WB detected in: Mouse liver tissue. All lanes: RASSF7 antibody at 4.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 37, 35 kDa. Observed band size: 40 kDa.
Immunofluorescence staining of HepG2 cells with PACO58468 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Negatively regulates stress-induced JNK activation and apoptosis by promoting MAP2K7 phosphorylation and inhibiting its ability to activate JNK. Following prolonged stress, anti-apoptotic effect stops because of degradation of RASSF7 protein via the ubiquitin-proteasome pathway. Required for the activation of AURKB and chromosomal congression during mitosis where it stimulates microtubule polymerization.
Synonyms:
Ras association domain-containing protein 7 (HRAS1-related cluster protein 1), RASSF7, C11orf13 HRC1
UniProt Protein Function:
HRC1: 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 11p15.5Molecular Function: protein binding
