The RASSF3 Polyclonal Antibody (PACO59201) is specifically designed for research involving RASSF3, a tumor suppressor protein involved in cell proliferation and apoptosis. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RASSF3 protein, this antibody enables the detection and analysis of RASSF3 in various cell types, making it an excellent tool for studies in cancer biology and tumor development.RASSF3, a member of the Ras association domain family, is known to play a pivotal role in regulating cell growth and promoting programmed cell death. Its involvement in various signaling pathways highlights its potential significance in cancer progression and metastasis.
Research into the function of RASSF3 is crucial for understanding its role in tumor suppression and identifying potential therapeutic targets for cancer treatment.By utilizing the RASSF3 Polyclonal Antibody (PACO59201), researchers can further investigate the mechanisms underlying RASSF3-mediated tumor suppression and explore its therapeutic potential in cancer therapy. This antibody provides a valuable tool for studying the complex signaling networks that contribute to cancer development and progression, ultimately leading to advancements in cancer research and therapy.
IHC image of PACO59201 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO59201 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
cytosol, plasma membrane, identical protein binding
Synonyms:
Ras association domain-containing protein 3, RASSF3
UniProt Protein Function:
RASSF3: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 12q14.2Cellular Component: cytoplasm; plasma membraneMolecular Function: identical protein binding; protein binding
UniProt Protein Details:
NCBI Summary:
The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]
