The RasGRP1 Polyclonal Antibody (CAB10495) is a valuable tool for researchers studying RasGRP1, a guanine nucleotide exchange factor involved in Ras signaling pathways. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to RasGRP1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in signaling pathways, cancer biology, and cell proliferation.
RasGRP1 is a key player in the regulation of cell growth, differentiation, and apoptosis, making it a promising target for research in cancer and other diseases. Its involvement in Ras signaling cascades suggests its potential as a therapeutic target for diseases driven by aberrant Ras activation. By understanding the function and regulation of RasGRP1, researchers can gain insight into the mechanisms underlying diseases such as cancer and develop targeted therapies to effectively combat them.
Product Name:
RASGRP1 Rabbit Polyclonal Antibody
SKU:
CAB10495
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2).
This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).
Purification Method:
Affinity purification
Gene ID:
10125
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using RASGRP1 Rabbit pAb (CAB10495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
