The RASA1 Monoclonal Antibody is a valuable tool for researchers studying RASA1, a critical regulator of cell signaling pathways involved in vascular development and tumor suppression. This antibody, produced through a monoclonal process, exhibits high specificity and sensitivity for detecting RASA1 protein in human samples, making it ideal for use in applications such as Western blotting and immunohistochemistry.RASA1, also known as p120RasGAP, acts as a negative regulator of Ras signaling, playing a key role in controlling cell growth, differentiation, and migration. Mutations in the RASA1 gene are associated with vascular disorders such as capillary malformation-arteriovenous malformation (CM-AVM) syndrome, highlighting its importance in vascular biology and disease.
By targeting the RASA1 protein, researchers can gain valuable insights into the mechanisms underlying vascular development and tumorigenesis, with potential implications for therapeutic intervention in vascular malformations and cancers. The RASA1 Monoclonal Antibody offers a reliable tool for exploring the functions of RASA1 in various cellular contexts, advancing our understanding of its role in health and disease.
Product Name:
RASA1 Monoclonal Antibody
SKU:
CAB4876
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RASA1 (P20936).
Sequence:
KSGS YLIR ESDR RPGS FVLS FLSQ MNVV NHFR IIAM CGDY YIGG RRFS SLSD LIGY YSHV SCLL KGEK LLYP VAPP EPVE DRRR VRAI LPYT KVPD TDEI S
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
Purification Method:
Affinity purification
Gene ID:
5921
Clone Number:
ARC2730
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using (CAB4876) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
