The Raptor Polyclonal Antibody (CAB21755) is a vital tool for studying Raptor, a key protein involved in the regulation of cell growth and metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting Raptor in human samples, making it an essential component in Western blot experiments. By binding to Raptor, researchers can analyze its expression and function in various cell types, offering valuable insights into cellular signaling pathways and potential therapeutic targets.
Raptor is a crucial component of the mTORC1 complex, which plays a central role in coordinating cellular nutrient sensing and growth. Dysregulation of mTORC1 signaling has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders. By investigating the role of Raptor in these pathways, researchers can uncover new potential treatments for these conditions, ultimately advancing our understanding of human health and disease.
Product Name:
Raptor Polyclonal Antibody
SKU:
CAB21755
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 888-1013 of human Raptor (NP_065812.1).
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
57521
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Raptor antibody (CAB21755) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
