The RapGEF2 Polyclonal Antibody (PACO60460) is a valuable tool for researchers studying RapGEF2, a signaling protein involved in various cellular processes, including cell growth, differentiation, and migration. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding to the RapGEF2 protein, this antibody allows for reliable detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and cancer research.RapGEF2, also known as Rap guanine nucleotide exchange factor 2, is a key player in intracellular signaling pathways that regulate cell behavior and function.
Its involvement in processes such as cell adhesion and cytoskeletal dynamics underscores its importance in cellular physiology and disease development. Research into the role of RapGEF2 is crucial for gaining insights into conditions like cancer, neurological disorders, and cardiovascular diseases, where dysregulation of signaling pathways can have profound consequences.By utilizing the RapGEF2 Polyclonal Antibody (PACO60460), scientists can delve deeper into the functions and mechanisms of RapGEF2, paving the way for potential therapeutic interventions targeting this protein in the treatment of various diseases.
Antibody Name:
RAPGEF2 Antibody (PACO60460)
Antibody SKU:
PACO60460
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Rap guanine nucleotide exchange factor 2 protein (1393-1498AA)
Immunofluorescence staining of Hela cells with PACO60460 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-α signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.
RAPGEF2: Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. Interacts with MAGI2. Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.Protein type: GEFs, Ras; Motility/polarity/chemotaxis; GEFsChromosomal Location of Human Ortholog: 4q32.1Cellular Component: apical plasma membrane; cell soma; cytoplasm; cytosol; endocytic vesicle; intercellular junction; late endosome; membrane; neuron projection; perinuclear region of cytoplasm; plasma membrane; protein complex; synapse; tight junctionMolecular Function: beta-1 adrenergic receptor binding; cAMP binding; cGMP binding; GTPase activator activity; PDZ domain binding; protein binding; Rap guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity; signal transducer activity; WW domain bindingBiological Process: blood vessel development; brain-derived neurotrophic factor receptor signaling pathway; cAMP-mediated signaling; forebrain neuron development; G-protein coupled receptor protein signaling pathway; MAPKKK cascade; microvillus biogenesis; negative regulation of cell proliferation; negative regulation of dendrite morphogenesis; negative regulation of melanin biosynthetic process; neurite development; neuron migration; neuropeptide signaling pathway; positive regulation of GTPase activity; positive regulation of protein binding; positive regulation of protein kinase activity; Rap protein signal transduction; regulation of synaptic plasticity; small GTPase mediated signal transduction; ventricular system development
UniProt Protein Details:
NCBI Summary:
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
