The RAPGEF1 Antibody (CAB16050) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, has high specificity for RAPGEF1 in human samples and has been validated for use in Western blot applications. By binding specifically to the RAPGEF1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in cell signaling, molecular biology, and drug development.RAPGEF1, also known as Rap guanine nucleotide exchange factor 1, is involved in regulating cellular processes such as cell growth, differentiation, and migration through its role in activating small GTPases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAPGEF1 Antibody
SKU:
CAB16050
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C3G, GRF2, RAPGEF1
Positive Sample:
Mouse liver
Cellular Localization:
Early Endosome.
Calculated MW:
121kDa
Observed MW:
120kDa
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
Purification Method
Affinity purification
Gene ID
2889
RRID
AB_2763489
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using RAPGEF1 Rabbit pAb (CAB16050) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using RAPGEF1 Rabbit pAb (CAB16050) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
