The RAP1B Polyclonal Antibody is a specialized tool for researchers studying RAP1B, a small GTPase protein involved in various cellular processes such as cell adhesion, migration, and signaling. This antibody, produced in rabbits, exhibits high reactivity with human and mouse samples and is validated for use in Western blot applications.By binding specifically to the RAP1B protein, this antibody allows for the detection and analysis of RAP1B levels in different cell types, making it a valuable asset for studies in cell biology, cancer research, and signal transduction pathways.
RAP1B is known to regulate cell adhesion and migration, making it a key player in cancer progression and metastasis. Understanding the function of RAP1B is crucial for developing targeted therapies for cancer and other diseases.Overall, the RAP1B Polyclonal Antibody offers researchers a reliable tool for investigating the role of RAP1B in cellular processes and disease mechanisms, paving the way for new insights and potential therapeutic approaches.
Product Name:
RAP1B Polyclonal Antibody
SKU:
CAB24472
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 81-181 of human RAP1B (NP_056461.1).
Sequence:
VYSI TAQS TFND LQDL REQI LRVK DTDD VPMI LVGN KCDL EDER VVGK EQGQ NLAR QWNN CAFL ESSA KSKI NVNE IFYD LVRQ INRK TPVP GKAR KKSS C
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:
RAP1B; K-REV; RAL1B; ras-related protein Rap-1b
Positive Sample:
Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cell junction, Cell membrane, Cytoplasm, cytosol.
Calculated MW:
15kDa/16kDa/18kDa/20kDa
Observed MW:
23kDa
This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.
Purification Method:
Affinity purification
Gene ID:
5908
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain, using RAP1B Rabbit pAb (CAB24472) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
