The Rap1A Polyclonal Antibody (CAB0975) is a powerful tool for researchers studying the Rap1A protein, a small GTPase involved in cell signaling pathways and regulation of cell adhesion and migration. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blot applications.Rap1A is a crucial mediator of various cellular processes, including cell adhesion, migration, and proliferation, as well as vesicle trafficking and signaling cascades. Aberrant activation or dysregulation of Rap1A has been implicated in diseases such as cancer and cardiovascular disorders, making it a valuable target for research in these fields.
By enabling the detection and analysis of Rap1A protein expression in different cell types, the Rap1A Polyclonal Antibody facilitates investigations into the functional role of Rap1A in normal physiological processes and disease pathogenesis. Its versatility and reliability make it a valuable asset for studies exploring the molecular mechanisms underlying cellular signaling pathways and potential therapeutic interventions targeting Rap1A-mediated pathways.
Product Name:
Rap1A Rabbit Polyclonal Antibody
SKU:
CAB0975
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 39-138 of human Rap1A (NP_002875.1).
Cell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region.
Calculated MW:
21kDa
Observed MW:
21kDa
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5906
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Rap1A Rabbit pAb (CAB0975) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
