The RANBP3L Polyclonal Antibody (PAC061478) is an essential tool for researchers studying the function of RANBP3L, a key protein involved in nucleocytoplasmic transport and cell division. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for experiments such as Western blotting.RANBP3L, also known as Ran-binding protein 3-like, is crucial for maintaining proper cellular functions through its role in regulating the transport of molecules between the nucleus and cytoplasm.
Dysregulation of RANBP3L has been associated with various diseases, including cancer and neurological disorders, underscoring the importance of studying its function in different contexts.By using the RANBP3L Polyclonal Antibody, researchers can effectively detect and analyze RANBP3L protein expression in various cell types, providing valuable insights into its role in cellular processes. This antibody is a valuable tool for advancing research in fields such as molecular biology, cell biology, and disease pathology.
IHC image of PACO61478 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with PACO61478 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Nuclear export factor for BMP-specific SMAD1/5/8 that plays a critical role in terminating BMP signaling and regulating mesenchymal stem cell differentiation by blocking osteoblast differentiation to promote myogenic differention. Directly recognizes dephosphorylated SMAD1/5/8 and mediates their nuclear export in a Ran-dependent manner.
Synonyms:
Ran-binding protein 3-like, RANBP3L
UniProt Protein Function:
RANBP3L: 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 5p13.2Cellular Component: cytosol; nucleusMolecular Function: Ran GTPase bindingBiological Process: positive regulation of protein export from nucleus
