The RAN Rabbit Monoclonal Antibody (CAB4374) is a cutting-edge tool for researchers studying the RAN protein, a key player in nucleocytoplasmic transport and cell cycle regulation. This antibody, developed using advanced monoclonal antibody technology in rabbits, displays high specificity and sensitivity for RAN in human samples, making it an excellent choice for a wide range of applications in molecular biology and cell biology research.RAN is a small GTPase involved in the transport of proteins and RNA through the nuclear pore complex, as well as in the regulation of mitotic spindle assembly during cell division. Dysregulation of RAN has been implicated in various diseases, including cancer and neurodegenerative disorders, underscoring the importance of studying this critical protein.
By targeting RAN with this specific monoclonal antibody, researchers can accurately detect and analyze RAN expression and localization in different cell types and experimental conditions. This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, enabling detailed investigation of RAN biology.Overall, the RAN Rabbit Monoclonal Antibody (CAB4374) is a valuable tool for researchers interested in elucidating the functions of RAN in health and disease, with potential implications for the development of novel therapeutic strategies targeting RAN-associated pathways.
Product Name:
Ran Rabbit Monoclonal Antibody
SKU:
CAB4374
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 117-216 of human Ran (P62826).
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Purification Method:
Affinity purification
Gene ID:
5901
Clone Number:
ARC0986
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (CAB4374) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
