The RAN Polyclonal Antibody (CAB0976) is an essential tool for researchers studying the RAN protein, a key player in nucleocytoplasmic transport and cell cycle progression. This antibody, derived from rabbits, has high specificity and sensitivity for detecting RAN in human samples and is validated for use in Western blot applications. By binding specifically to the RAN protein, this antibody enables precise detection and analysis in a variety of cell types, making it the perfect choice for studies in cell biology and cancer research.
RAN, a small GTPase, is crucial for the transport of proteins and RNA through the nuclear pore complex, as well as for the regulation of mitotic spindle assembly during cell division. Dysregulation of RAN expression or activity has been implicated in various diseases, including cancer and neurodegenerative disorders. Therefore, a thorough understanding of RAN function is essential for the development of targeted therapies aimed at correcting RAN-related abnormalities and improving patient outcomes.
Product Name:
Ran Rabbit Polyclonal Antibody
SKU:
CAB0976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human Ran (NP_006316.1).
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Purification Method:
Affinity purification
Gene ID:
5901
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Ran antibody (CAB0976) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
