The RAMP1 Polyclonal Antibody (CAB6447) is a valuable tool for researchers studying the RAMP1 protein, a key regulator of G protein-coupled receptor function. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RAMP1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of neuroscience and cardiovascular research.
RAMP1 is known to play a crucial role in modulating the activity of specific G protein-coupled receptors, leading to effects on various physiological processes including blood pressure regulation and pain perception. Research on RAMP1 is essential for gaining insights into conditions such as hypertension, migraine, and neuropathic pain. By elucidating the functions of RAMP1, researchers can potentially identify novel therapeutic targets for the treatment of these disorders.
Product Name:
RAMP1 Rabbit Polyclonal Antibody
SKU:
CAB6447
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 21-120 of human RAMP1 (NP_005846.1).
Sequence:
CPIS GRAV RDPP GS
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:
RAMP1
Positive Sample:
Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Membrane, Single-pass type I membrane protein.
Calculated MW:
17kDa
Observed MW:
17kDa
The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10267
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse brain, using RAMP1 antibody (CAB6447) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
