null

[KO Validated] RAG2 Rabbit Polyclonal Antibody (CAB5626)

SKU:
CAB5626
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • KO Validated Antibodies 1 Anti-RAG2 Antibody CAB5626KO Validated
  • KO Validated Antibodies 1 Anti-RAG2 Antibody CAB5626KO Validated
  • KO Validated Antibodies 1 Anti-RAG2 Antibody CAB5626KO Validated
  • KO Validated Antibodies 1 Anti-RAG2 Antibody CAB5626KO Validated
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] RAG2 Rabbit Polyclonal Antibody
SKU:CAB5626
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 351-527 of human RAG2 (NP_000527.2).
Sequence:AEDD TNEE QTTF TNSQ TSTE DPGD STPF EDSE EFCF SAEA NSFD GDDE FDTY NEDD EEDE SETG YWIT CCPT CDVD INTW VPFY STEL NKPA MIYC SHGD GHWV HAQC MDLA ERTL IHLS AGSN KYYC NEHV EIAR ALHT PQRV LPLK KPPM KSLR KKGS GKIL TPAK KSFL RRLF D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RAG-2; G2
Positive Sample:Raji,Jurkat,293T,HepG2
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:59kDa
Observed MW:59kDa

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Purification Method:Affinity purification
Gene ID:5897
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] RAG2 Rabbit pAb (CAB5626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Related Products