The RAG2 Polyclonal Antibody (CAB12488) is a valuable tool for researchers studying RAG2, a key protein involved in the process of V(D)J recombination in the immune system. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the RAG2 protein, this antibody enables the detection and analysis of RAG2 in various cell types, making it ideal for studies in immunology and hematopoiesis.RAG2 is essential for the generation of diverse antigen receptor genes in B and T cells, and abnormalities in its function can lead to immune deficiencies and autoimmune diseases.
Research on RAG2 is crucial for understanding the mechanisms of immune system development and function. The RAG2 Polyclonal Antibody is a valuable tool for investigating the role of RAG2 in normal and pathological immune responses, making it a vital resource for studies in immunology and autoimmunity.
Product Name:
RAG2 Rabbit Polyclonal Antibody
SKU:
CAB12488
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_001230715.1).
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
Purification Method:
Affinity purification
Gene ID:
5897
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RAG2 antibody (CAB12488) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
