The RAG1 Polyclonal Antibody (CAB12646) is a valuable tool for researchers studying RAG1, an essential protein involved in V(D)J recombination, a process crucial for generating diverse antigen receptor genes in immune cells. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RAG1 protein, this antibody enables the detection and analysis of RAG1 expression in various cell types, making it ideal for investigations in immunology and hematopoiesis research.RAG1, a key component of the RAG complex, plays a pivotal role in initiating the recombination process that generates functional T and B cell receptors.
Understanding the regulation and activity of RAG1 is essential for unraveling the mechanisms underlying immune cell development and function. Research on RAG1 is particularly relevant for studies on immune deficiencies, lymphoid malignancies, and autoimmune disorders, as dysregulation of V(D)J recombination can lead to serious immune dysfunctions. The RAG1 Polyclonal Antibody provides researchers with a reliable tool for investigating the functions and regulatory mechanisms of RAG1 in health and disease.
Product Name:
RAG1 Rabbit Polyclonal Antibody
SKU:
CAB12646
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1 (NP_000439.1).
The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Purification Method:
Affinity purification
Gene ID:
5896
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse thymus, using RAG1 Rabbit pAb (CAB12646) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
