The RAD54B Polyclonal Antibody (CAB16208) is a valuable tool for researchers studying the RAD54B protein, a key player in DNA repair and recombination processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to RAD54B, this antibody enables the detection and analysis of the protein in various cell types, making it ideal for investigations in genetics, molecular biology, and oncology research.
RAD54B is known for its role in DNA double-strand break repair and homologous recombination, making it crucial for maintaining genomic stability and preventing mutations that can lead to cancer and other genetic disorders. Research on RAD54B is essential for understanding the mechanisms underlying DNA repair pathways and developing targeted therapies for diseases linked to DNA damage. The RAD54B Polyclonal Antibody is a valuable tool for unraveling the functions and interactions of RAD54B in these contexts.
Product Name:
RAD54B Rabbit Polyclonal Antibody
SKU:
CAB16208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 781-910 of human RAD54B (NP_036547.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RDH54; RAD54B
Positive Sample:
HeLa,Jurkat,DU145,HT-29,mouse testis
Conjugate:
Unconjugated
Cellular Localization:
nucleus
Calculated MW:
103kDa
Observed MW:
103kDa
The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
Purification Method:
Affinity purification
Gene ID:
25788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RAD54B Rabbit pAb (CAB16208) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
