The RAD52 Rabbit Monoclonal Antibody (CAB5186) is a highly specific and sensitive tool for researchers studying RAD52, a key player in DNA repair and recombination processes. This antibody, produced using rabbit monoclonal technology, is optimized for detecting RAD52 protein in human samples and is suitable for use in Western blot assays.RAD52 is essential for maintaining genome stability and promoting DNA repair through homologous recombination, making it a critical factor in preventing genomic instability and tumorigenesis. By targeting RAD52, researchers can gain insights into the mechanisms underlying DNA repair pathways and identify potential therapeutic targets for cancer treatment.
The RAD52 Rabbit Monoclonal Antibody (CAB5186) enables precise detection and quantification of RAD52 protein levels in various cell types, offering valuable information for studies in molecular biology, cancer research, and genomics. Its high specificity and sensitivity make it an invaluable tool for elucidating the role of RAD52 in DNA repair mechanisms and developing novel strategies for cancer therapy.
Product Name:
Rad52 Rabbit Monoclonal Antibody
SKU:
CAB5186
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 319-418 of human Rad52 (P43351).
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Purification Method:
Affinity purification
Gene ID:
5893
Clone Number:
ARC1183
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Rad52 Rabbit mAb (CAB5186) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
