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Rad52 Polyclonal Antibody (CAB21587)

SKU:
CAB21587
€129
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Description

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Product Name:Rad52 Polyclonal Antibody
SKU:CAB21587
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human Rad52 (NP_602296.2).
Sequence:GYGV SEGL KSKA LSLE KARK EAVT DGLK RALR SFGN ALGN CILD KDYL RSLN KLPR QLPL EVDL TKAK RQDL EPSV EEAR YNSC RPNM ALGH PQLQ QVTS PSRP SHAV IPAD QDCS SRSL SSSA VESE ATHQ RKLR QKQL QQQF RERM EKQQ VRVS TPSA EKSE AAPP APPV THST PVTV SEPL LEKD FLAG VTQE LIKT LEDN SEKW AVTP DAGD GVVK PSSR ADPA QTSD TLAL NNQM VTQN RTPH SVCH QKPQ AKSG SWDL QTYS ADQR TTGN WESH RKSQ DMKK RKYD PS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RAD52; DNA repair protein RAD52 homolog; Rad52
Positive Sample:Rat thymus
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:46kDa
Observed MW:46kDa

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Purification Method:Affinity purification
Gene ID:5893
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Rat thymus using Rad52 Rabbit pAb(CAB21587) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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