The RAD52 Polyclonal Antibody (CAB3077) is a valuable tool for research involving RAD52, a key player in DNA repair processes. This antibody, raised in rabbits, is highly specific for human RAD52 samples and is validated for use in a variety of applications, including Western blotting. By binding to RAD52 protein, this antibody allows for the detection and analysis of RAD52 in different cellular contexts, making it an ideal choice for studies in DNA repair mechanisms and cancer research.RAD52 is essential for homologous recombination, a process crucial for repairing double-strand breaks in DNA and maintaining genome stability.
Dysregulation of RAD52 has been linked to various diseases, including cancer and genetic disorders. Understanding the role of RAD52 in DNA repair pathways is important for developing targeted therapies and improving cancer treatment strategies.By utilizing the RAD52 Polyclonal Antibody (CAB3077), researchers can gain valuable insights into the functions of RAD52 and its implications in DNA repair mechanisms and disease progression. This antibody offers a reliable and specific tool for studying RAD52 expression and activity, advancing research in the fields of genetics, cancer biology, and molecular biology.
Product Name:
Rad52 Rabbit Polyclonal Antibody
SKU:
CAB3077
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human Rad52 (NP_602296.2).
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Purification Method:
Affinity purification
Gene ID:
5893
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Rad52 antibody (CAB3077) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
