The RAD51D Polyclonal Antibody (CAB7534) is a valuable tool for researchers studying RAD51D, a protein involved in DNA repair mechanisms and genome stability. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RAD51D protein, it enables precise detection and analysis in a variety of cell types, making it well-suited for investigations in the fields of genetics, oncology, and molecular biology.RAD51D is a crucial component of the homologous recombination pathway, playing a key role in repairing DNA double-strand breaks and maintaining genomic integrity. Dysregulation of RAD51D has been linked to various genetic disorders and cancer development, highlighting its importance in cellular function.
Researchers studying DNA repair mechanisms or genetic disorders will benefit from the use of this antibody to further understand the role of RAD51D in normal and diseased cells.By uncovering the mechanisms by which RAD51D operates in DNA repair and genome stability, researchers can potentially identify new therapeutic targets for diseases associated with defective DNA repair, such as cancer. The RAD51D Polyclonal Antibody (CAB7534) provides a reliable tool for investigating the functions of RAD51D and advancing scientific knowledge in the field of genetics and oncology.
Product Name:
Rad51D Rabbit Polyclonal Antibody
SKU:
CAB7534
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human Rad51D (NP_002869.3).
Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, telomere.
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene.
Purification Method:
Affinity purification
Gene ID:
5892
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Rad51D antibody (CAB7534) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
