The RAD51 Rabbit Monoclonal Antibody (CAB2829) is a high-quality research tool designed for the study of RAD51, a key player in DNA repair and recombination processes. This antibody, produced in rabbits, is specifically tailored for use in immunofluorescence and Western blot techniques, providing accurate and reliable detection of RAD51 protein levels in various sample types.RAD51 is a crucial protein involved in the repair of DNA double-strand breaks, playing a vital role in maintaining genomic stability and preventing the accumulation of mutations. Dysregulation of RAD51 function has been linked to various diseases, including cancer, highlighting its importance in maintaining cellular integrity.
By utilizing the RAD51 Rabbit Monoclonal Antibody (CAB2829), researchers can gain insights into the expression and localization of RAD51 within cells, facilitating further investigation into its role in DNA repair mechanisms. This antibody is a valuable tool for studies in cancer biology, genetics, and developmental biology, providing essential information for the development of targeted therapies aimed at enhancing DNA repair processes for therapeutic purposes.
Product Name:
Rad51 Rabbit Monoclonal Antibody
SKU:
CAB2829
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad51 (Q06609).
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5888
Clone Number:
ARC0764
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Rad51 Rabbit mAb (CAB2829) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
