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Rad51 Rabbit Monoclonal Antibody (CAB2829)

SKU:
CAB2829
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Rad51 Rabbit Monoclonal Antibody
SKU:CAB2829
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad51 (Q06609).
Sequence:MAMQ MQLE ANAD TSVE EESF GPQP ISRL EQCG INAN DVKK LEEA GFHT VEAV AYAP KKEL INIK GISE AKAD KILA EAAK LVPM GFTT ATEF HQRR SEII
Tested Applications:WB ChIP ELISA
Recommended Dilution:WB,1:500 - 1:2000 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930; Rad51
Positive Sample:HeLa,MCF7,Mouse testis,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion matrix, Nucleus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region.
Calculated MW:37kDa
Observed MW:37kDa

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5888
Clone Number:ARC0764
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Rad51 Rabbit mAb (CAB2829) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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