The RAD50 Polyclonal Antibody (CAB3078) is a valuable tool for researchers studying the RAD50 protein, a key player in DNA repair pathways and genome stability maintenance. Raised in rabbits, this antibody is highly specific for human RAD50 samples and has been validated for use in Western blot applications.RAD50 is a member of the MRN complex, which is essential for detecting and repairing DNA double-strand breaks. Dysregulation of RAD50 has been implicated in various diseases, including cancer and genetic disorders.
By targeting the RAD50 protein, researchers can gain insight into the mechanisms underlying these conditions and potentially identify new therapeutic targets.This antibody enables precise detection and analysis of RAD50 in different cell types, making it a versatile tool for studies in genetics, oncology, and molecular biology. By understanding the role of RAD50 in DNA repair and genome maintenance, researchers can uncover new avenues for treating diseases characterized by genomic instability.
Product Name:
Rad50 Rabbit Polyclonal Antibody
SKU:
CAB3078
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad50 (NP_005723.2).
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Purification Method:
Affinity purification
Gene ID:
10111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Rad50 Rabbit pAb (CAB3078) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
