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Rad50 Rabbit Polyclonal Antibody (CAB3078)

SKU:
CAB3078
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-Rad50 Antibody CAB3078
  • Cell Cycle Antibodies 1 Anti-Rad50 Antibody CAB3078
  • Cell Cycle Antibodies 1 Anti-Rad50 Antibody CAB3078
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Rad50 Rabbit Polyclonal Antibody
SKU:CAB3078
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad50 (NP_005723.2).
Sequence:MSRI EKMS ILGV RSFG IEDK DKQI ITFF SPLT ILVG PNGA GKTT IIEC LKYI CTGD FPPG TKGN TFVH DPKV AQET DVRA QIRL QFRD VNGE LIAV QRSM
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:NBSLD; RAD502; hRad50; Rad50
Positive Sample:HeLa,K-562,U-937,Mouse testis,Mouse thymus,Rat testis
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus, telomere.
Calculated MW:154kDa
Observed MW:154kDa

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Purification Method:Affinity purification
Gene ID:10111
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Rad50 Rabbit pAb (CAB3078) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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