The Rad50 Polyclonal Antibody (CAB0182) is a research tool specifically designed for studying Rad50, a key protein involved in DNA repair and maintenance. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting.Rad50 is an essential part of the MRN complex, which is responsible for detecting and repairing double-strand breaks in DNA. Mutations in Rad50 have been linked to various genetic disorders and cancer development, making it a crucial target for research in the field of genomics and oncology.
Researchers can use this antibody to detect and analyze Rad50 levels in different cell types, allowing for a better understanding of its function in DNA repair pathways. By studying Rad50, scientists can potentially uncover new insights into disease mechanisms and develop targeted therapies for conditions related to DNA damage.
Product Name:
Rad50 Rabbit Polyclonal Antibody
SKU:
CAB0182
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Rad50 (NP_005723.2).
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Purification Method:
Affinity purification
Gene ID:
10111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Rad50 Rabbit pAb (CAB0182) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
