Rad23B Polyclonal Antibody (CAB21586)
- SKU:
- CAB21586
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
Description
| Product Name: | Rad23B Polyclonal Antibody |
| SKU: | CAB21586 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Rad23B (NP_002865.1). |
| Sequence: | TSST TTTV AQAP TPVP ALAP TSTP ASIT PASA TASS EPAP ASAA KQEK PAEK PAET PVAT SPTA TDST SGDS SRSN LFED ATSA LVTG QSYE NMVT EIMS M |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
| Synonyms: | P58; HR23B; HHR23B; Rad23B |
| Positive Sample: | HeLa, Jurkat, SKOV3, Mouse brain, Mouse testis, Mouse liver |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus. |
| Calculated MW: | 43kDa |
| Observed MW: | 53kDa |
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
| Purification Method: | Affinity purification |
| Gene ID: | 5887 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Rad23B Rabbit pAb (CAB21586) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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