The RAD18 Rabbit Monoclonal Antibody (CAB4339) is a valuable tool for researchers studying RAD18, a key protein involved in DNA damage repair and maintenance of genomic stability. This antibody, developed using rabbit monoclonal technology, is highly specific to human samples and has been validated for use in Western blotting applications.RAD18 plays a crucial role in the post-replication repair pathway, facilitating the recruitment of DNA repair factors to sites of DNA damage. Dysregulation of RAD18 has been linked to increased genomic instability and susceptibility to cancer development. By targeting RAD18 with this antibody, researchers can investigate its cellular functions in various biological contexts, including DNA repair mechanisms, cell cycle regulation, and the response to DNA-damaging agents.
With its high reactivity and specificity, the RAD18 Rabbit Monoclonal Antibody (CAB4339) is an essential tool for studies in molecular biology, cancer research, and genomics. By enabling the detection and analysis of RAD18 protein expression, this antibody contributes to a better understanding of the mechanisms underlying genome maintenance and the development of potential therapeutic strategies for cancer and other diseases related to DNA damage.
Product Name:
Rad18 Rabbit Monoclonal Antibody
SKU:
CAB4339
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad18 (Q9NS91).
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Purification Method:
Affinity purification
Gene ID:
56852
Clone Number:
ARC1058
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Rad18 Rabbit mAb (CAB4339) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
