The RAD18 Polyclonal Antibody (CAB13434) is a valuable tool for researchers studying RAD18, a protein involved in DNA repair and maintenance of genomic stability. This antibody, produced in rabbits, is highly specific for RAD18 in human samples and is validated for use in Western blot applications.RAD18 plays a crucial role in DNA damage response by promoting the replication of damaged DNA to prevent genomic instability. Dysregulation of RAD18 has been implicated in a variety of diseases, including cancer and neurodegenerative disorders.
By targeting RAD18 with this antibody, researchers can investigate its role in DNA repair processes and potential therapeutic interventions.With its high reactivity and specificity, the RAD18 Polyclonal Antibody is an essential tool for studies focusing on DNA repair mechanisms, genomic stability, and disease pathology. Its application in Western blotting provides a reliable method for detecting and analyzing RAD18 protein levels in various cell types, making it ideal for research in cancer biology, genetics, and molecular biology.
Product Name:
Rad18 Rabbit Polyclonal Antibody
SKU:
CAB13434
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3).
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Purification Method:
Affinity purification
Gene ID:
56852
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Rad18 antibody (CAB13434) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.