The RAD17 Polyclonal Antibody (CAB5359) is a valuable tool for researchers studying the RAD17 protein, a key player in the DNA damage response pathway. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to RAD17, this antibody allows for accurate detection and analysis of the protein in various cell types, making it an essential component in studies focused on DNA repair mechanisms and cell cycle regulation.RAD17, a checkpoint protein involved in sensing DNA damage and facilitating repair processes, is essential for maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer development.
Research on RAD17 has the potential to uncover novel therapeutic targets for cancer treatment and improve our understanding of how cells maintain genome integrity in the face of genotoxic stress.In conclusion, the RAD17 Polyclonal Antibody (CAB5359) is a reliable reagent that can enhance the quality and accuracy of experiments related to DNA damage response pathways and genomic stability, ultimately contributing to advancements in the fields of genetics, oncology, and molecular biology.
Product Name:
RAD17 Rabbit Polyclonal Antibody
SKU:
CAB5359
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CCYC; R24L; RAD24; HRAD17; RAD17SP; RAD17
Positive Sample:
SW480,BT-474,K-562,22Rv1,Raji
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
77kDa
Observed MW:
77kDa
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Purification Method:
Affinity purification
Gene ID:
5884
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RAD17 Rabbit pAb (CAB5359) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
