RAD17 Rabbit Polyclonal Antibody (CAB5359)
- SKU:
- CAB5359
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
Product Name: | RAD17 Rabbit Polyclonal Antibody |
SKU: | CAB5359 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1). |
Sequence: | KILY CKRA SLTE LDSP RLPS HLSE YERD TLLV EPEE VVEM SHMP GDLF NLYL HQNY IDFF MEID DIVR ASEF LSFA DILS GDWN TRSL LREY STSI ATRG VMHS NKAR GYAH CQGG GSSF RPLH KPQW FLIN KKYR ENCL AAKA LFPD FCLP ALCL QTQL LPYL ALLT IPMR NQAQ ISFI QDIG RLPL KRHF GRLK MEAL TDRE HGMI DPDS GDEA QLNG GHSA EESL GEPT QATV PETW SLPL SQNS ASEL PASQ PQPF SAQG DMEE NIII EDYE SDGT |
Tested Applications: | WB IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | CCYC; R24L; RAD24; HRAD17; RAD17SP; RAD17 |
Positive Sample: | SW480,BT-474,K-562,22Rv1,Raji |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 77kDa |
Observed MW: | 77kDa |
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Purification Method: | Affinity purification |
Gene ID: | 5884 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |