The Rabbit VWF (Von Willebrand Factor) ELISA Kit is a specialized assay meticulously developed to quantitatively detect Von Willebrand Factor (VWF) levels in various rabbit biological samples. VWF is crucial in hemostasis and plays a fundamental role in platelet function and blood clotting. Accurate measurement of VWF is essential for investigating various bleeding disorders, thrombotic diseases, and vascular health conditions in rabbits.
This ELISA kit is engineered to deliver exceptional sensitivity and specificity, ensuring precise and reproducible results in VWF quantification. Manufactured under rigorous quality control standards, the kit offers robust performance and user-friendly procedures, making it an excellent choice for researchers studying rabbit models of hemostasis, thrombosis, and vascular biology.
Product Name:
Rabbit VWF (Von Willebrand Factor) ELISA Kit
SKU:
AEES00578
Target:
Rabbit VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit VWF. You can calculate the concentration of Rabbit VWF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
88-103
92-109
Average (%)
96
95
100
1:4
Range (%)
85-98
86-99
96-111
Average (%)
91
93
103
1:8
Range (%)
88-102
89-104
89-103
Average (%)
93
96
97
1:16
Range (%)
87-99
88-103
94-108
Average (%)
94
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.64
7.28
35.58
3.51
7.6
33.41
Standard deviation
0.22
0.43
1.49
0.24
0.35
1.4
C V (%)
6.04
5.91
4.19
6.84
4.61
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit VWF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit VWF in samples. No significant cross-reactivity or interference between Rabbit VWF and analogues was observed.
