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Rabbit Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

SKU:
RBDL00083
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G1T1S9
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD106,INCAM-100,L1CAM
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rabbit Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

The Rabbit VCAM-1 (Vascular Cell Adhesion Molecule 1) ELISA Kit is specifically designed for the accurate measurement of VCAM-1 levels in rabbit serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.VCAM-1 is a key protein involved in the adhesion of leukocytes to the endothelium, playing a critical role in inflammation and immune response.

Dysregulation of VCAM-1 has been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and ease of use, the Rabbit VCAM-1 ELISA Kit is an essential tool for researchers interested in investigating the role of VCAM-1 in various physiological and pathological processes.

Product Name:Rabbit Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
Product Code:RBDL00083
Size:96 Assays
Target:VCAM1
Synonyms:CD106, INCAM-100, L1CAM
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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