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Rabbit Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

SKU:
RBDL00079
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04924
Sensitivity:
5.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
DIF,TNF-A,TNFSF2,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF,Tumor Necrosis Factor
Reactivity:
Rabbit
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Rabbit Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

The Rabbit Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit is specifically designed for the accurate and reliable detection of TNF-α levels in rabbit serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TNF-α is a key cytokine involved in inflammation, immune response, and the regulation of cell survival. Abnormal levels of TNF-α have been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions.

By measuring TNF-α levels, researchers can gain valuable insights into disease processes and potential therapeutic targets.With its user-friendly protocol and robust performance, the Rabbit TNF-α ELISA Kit is an essential tool for studying the role of TNF-α in disease pathogenesis and developing novel treatment strategies. Trust AssayGenie for high-quality ELISA kits that deliver accurate and reproducible results for your research needs.

Product Name:Rabbit Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
Product Code:RBDL00079
Size:96 Assays
Target:TNFa
Synonyms:DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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