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Rabbit Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU:
RBDL00073
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G1SV95
Sensitivity:
6.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B1,CED,DPD1,LAP,Camurati-Engelmann Disease,Latency-associated peptide
Reactivity:
Rabbit
Sample Type:
Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rabbit Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The Rabbit Transforming Growth Factor Beta-1 (TGF-β1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of TGF-β1 levels in rabbit serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it a valuable tool for various research applications.TGF-β1 is a key regulator of cell growth, differentiation, and development, playing a crucial role in immune response, tissue repair, and fibrosis.

Dysregulation of TGF-β1 signaling has been implicated in various diseases, including cancer, fibrosis, and autoimmune disorders, highlighting the importance of monitoring TGF-β1 levels for research and therapeutic purposes.With its advanced technology and easy-to-use protocol, the Rabbit TGF-β1 ELISA Kit from AssayGenie offers researchers a reliable method for studying TGF-β1 and its impact on various biological processes and disease pathways.

Product Name:Rabbit Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit
Product Code:RBDL00073
Size:96 Assays
Target:TGFb1
Synonyms:TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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