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Rabbit Tissue Factor (TF) ELISA Kit

SKU:
RBDL00071
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24055
Sensitivity:
23.1pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD142,FIII,F3,TFA,Thromboplastin,Coagulation Factor III
Reactivity:
Rabbit
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Tissue Factor (TF) ELISA Kit

The Rabbit Tissue Factor (TF) ELISA Kit is specifically designed for the precise measurement of tissue factor levels in rabbit serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research requirements.Tissue factor is a vital protein that plays a key role in the initiation of blood clotting and coagulation cascades. Dysregulation of tissue factor expression has been implicated in various diseases such as thrombosis, inflammation, and cancer.

As such, measuring tissue factor levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapies. With the Rabbit Tissue Factor (TF) ELISA Kit, researchers can accurately and efficiently quantify tissue factor levels in rabbit samples, facilitating in-depth investigations into the role of tissue factor in health and disease.

Product Name:Rabbit Tissue Factor (TF) ELISA Kit
Product Code:RBDL00071
Size:96 Assays
Target:TF
Synonyms:CD142, FIII, F3, TFA, Thromboplastin, Coagulation Factor III
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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