The Rabbit Thrombomodulin (TM) ELISA Kit is specifically designed for the precise measurement of thrombomodulin levels in rabbit serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.Thrombomodulin is a critical regulator of blood coagulation and inflammation, playing a key role in maintaining vascular integrity and preventing thrombosis. Dysregulation of thrombomodulin has been implicated in various diseases such as sepsis, thrombotic disorders, and cardiovascular diseases, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.
This ELISA kit provides researchers with the necessary tools to study thrombomodulin levels in rabbit models, contributing to a better understanding of its role in disease pathology and the development of novel therapeutic strategies. Invest in the Rabbit Thrombomodulin (TM) ELISA Kit for reliable and insightful research outcomes.
Product Name:
Rabbit Thrombomodulin (TM) ELISA Kit
Product Code:
RBDL00078
Size:
96 Assays
Target:
TM
Synonyms:
CD141, THBD, BDCA3, THRM, Fetomodulin
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
