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Rabbit Selectin, Platelet (SELP) ELISA Kit

SKU:
RBDL00069
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G1TBQ3
Sensitivity:
0.282ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62P,P-Selectin,PSEL,GMP140,LECAM3,GRMP,GMRP,PADGEM,Leukocyte-endothelial cell adhesion molecule 3,Platelet activation dependent granule-external membrane
Reactivity:
Rabbit
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Selectin, Platelet (SELP) ELISA Kit

The Rabbit Selectin Platelet SELP ELISA kit is a powerful tool for the precise measurement of selectin platelet SELP levels in rabbit samples. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes. Selectin platelet SELP is an important molecule involved in the adhesion of platelets to the endothelium, playing a critical role in processes such as inflammation, immune response, and thrombosis.

Its presence is indicative of platelet activation and endothelial dysfunction, making it a valuable biomarker for studying these processes and exploring potential therapeutic interventions. Overall, the Rabbit Selectin Platelet SELP ELISA kit is an indispensable resource for researchers seeking to gain insight into platelet function and endothelial interactions in rabbits, offering reliable and accurate data for advancing scientific knowledge and discoveries.

Product Name:Rabbit Selectin, Platelet (SELP) ELISA Kit
Product Code:RBDL00069
Size:96 Assays
Target:SELP
Synonyms:CD62P, P-Selectin, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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