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Rabbit Prolactin (PRL) ELISA Kit

SKU:
RBDL00063
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q28632
Sensitivity:
0.109ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LTH,Luteotropic Hormone
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Prolactin (PRL) ELISA Kit

The Rabbit Prolactin (PRL) ELISA Kit is a reliable and accurate tool for detecting prolactin levels in rabbit serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for a variety of research purposes.Prolactin is a key hormone involved in various physiological processes, including lactation, reproduction, and metabolism. Dysregulation of prolactin levels is associated with several health conditions, making it an important biomarker for studying these diseases and potential treatment options.

Overall, the Rabbit Prolactin (PRL) ELISA Kit provides researchers with a valuable tool for studying the role of prolactin in various biological processes and diseases, ultimately contributing to advancements in the field of hormone research.

Product Name:Rabbit Prolactin (PRL) ELISA Kit
Product Code:RBDL00063
Size:96 Assays
Target:PRL
Synonyms:LTH, Luteotropic Hormone
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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